2.4.1.198: phosphatidylinositol N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about phosphatidylinositol N-acetylglucosaminyltransferase, go to the full flat file.

Reaction

Synonyms

acetyl-D-glucosaminyltransferase, uridine diphosphoacetylglucosamine alpha1,6-, afpig-a, Down syndrome critical region protein 5, DR437w, Dscr5, glycosylphosphatidylinositol N-acetylglucosaminyltrans�ferase complex, glycosylphosphatidylinositol-N-acetylglucosaminyltransferase, GntA, GPI-GlcNAc transferase, GPI-GnT, GPI-N-acetylglucosaminyltransferase, GPI-N-acetylglucosaminyltransferase complex, GPI1, GPI15, GPI19, GPI2, GPI3, phosphatidylinositol N-acetylglucosaminyltransferase subunit A, phosphatidylinositol/UDP-GlcNAc:GlcNAc transferase, PIG-A, PigA, UDP-N-acetyl-D-glucosamine:phosphatidylinositol N-acetyl-D-glucosaminyltransferase, uridine diphosphoacetylglucosamine alpha1,6-acetyl-D-glucosaminyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.198 phosphatidylinositol N-acetylglucosaminyltransferase

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Results	in table
5	Activating Compound
2988	AA Sequence
33	Application
1	CAS Registry Number
22	Cloned(Commentary)
1	Cofactor
1	Crystallization (Commentary)
546	Disease/ Diagnostics
2	Expression
34	General Information
5	Inhibitors
28	Localization
6	Metals/Ions
9	Molecular Weight [Da]
33	Natural Substrates/ Products (Substrates)
82	Organism
1	Pathway
9	pH Optimum
1	Posttranslational Modification
18	Protein Variants
4	Purification (Commentary)
1	Reaction
1	Reaction Type
67	Reference
17	Source Tissue
1	Specific Activity [micromol/min/mg]
82	Substrates and Products (Substrate)
8	Subunits
113	Synonyms
1	Systematic Name
10	Temperature Optimum [°C]

Crystallization

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Crystallization on EC 2.4.1.198 - phosphatidylinositol N-acetylglucosaminyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant PigA in apoform and in complex with cofactor FAD or L-proline, sitting drop vapor diffusion method, mixing of 0.001 ml of 15 mg/ml protein in 100 mm NaCl and 50 mm Tris·HCl, pH 8.0, with 0.001 ml of reservoir solution containing 27% PEG 600, and 0.1 M Na-HEPES, pH 7.5, for the complex crystals FAD is added in a molar ratio of 3:1 ratio to the protein solution, and mixed with a reservoir solution containing 40% ethylene glycol and 0.1 M Na-acetate, pH 5.0; or 1.7 M (NH4)2SO4, 0.2 M NaCl, and 0.1 M sodium cacodylate, pH 6.5, for the proline-complex crystal the protein solution contains 7 mM L-proline in addition to FAD and crown ether, and the reservoir contains 1.9 M sodium malonate, pH 6.0. The Pro-Gly complex crystal is obtained by including 5 mM L-prolylglycine in the E244A protein solution, with a reservoir of 1.6 M (NH4)2SO4, 2% PEG 400, and 0.1 M citric acid, pH 4.0, X-ray diffraction structure determination and analysis at 1.3-2.55 A resolution, molecular replacement using the structure of butyryl-CoA dehydrogenase (PDB ID 4L1F) as a search model

Serratia marcescens

Q5W254

759061