This is an abbreviated version! For detailed information about alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, go to the full flat file.
acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-, alpha-1,3-mannosyl-glycoprotein beta-1,2-N-acetylglucosaminyltransferase, alpha3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, AtGnTI, beta-1-2 GlcNAc transferase, ChGnTI, EC 2.4.1.51, GlcNAc transferase I, GlcNAc-T I, GlcNAc-TI, GlcNAcT-I, gly-12, GLY-13, gly-14, GnT I, GnT-I, GnT1, GnTI, Golgi N-glycan branching N-acetylglucosaminyltransferase I, HuGnTI, MGAT1, N-acetylglucosaminyltransferase I, N-acetylglucosaminyltransferases I, N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I, NT, pigGnT-I, PtGnTI, TbGnTI, UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase, UDP-GlcNAc:alpha3-D-mannoside beta1,2-N-acetylglucosaminyltransferase I, UDP-GlcNac:alpha3-D-mannoside beta1,2-Nacetylglucosaminyltransferase I, UDP-GlcNAc:alpha3-D-mannoside beta1-2-N-acetylglucosaminyltransferase I, UDP-N-acetyl-D-glucosamine:glycoprotein (N-acetyl-D-glucosamine to alpha-D-mannosyl-1,3-(R1)-beta-D-mannosyl-R2) beta-1,2-N-acetyl-D-glucosaminyltransferase, UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-1,3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-acetylglucosaminyl:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, UDP-N-GlcNAc:alpha3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I, uridine diphosphoacetylglucosamine-alpha-1,3-mannosylglycoprotein beta-1,2-N-acetylglucosaminyltransferase
expression of the enzymes catalytic domain in Escherichia coli strain BL21, unmodified catalytic domain and His-tagged at the C-terminal and at the N-terminal end
expression of the histidine-tagged catalytic domain in Escherichia coli which is supplemented with mammalian tRNA codons and with mutations to improve intracellular disulfide bond formation
expression of YFP or CFP fusion enzyme of Arabidopsis and human N-acetylglucosaminyltransferase I, and the chimeric enzyme of Arabidopsis CTS region and human catalytic domain in mutant cgl1-1 Arabidopsis (lacking N-acetylglucosaminyltransferase I), transformed by Agrobacterium tumefaciens
functional expression of c-Myc-tagged full length clone and chimera TfR/GnT1myc and exchange mutants of the latter in CHO Lec 1 cells, lacking GnT-1 activity
functional expression of isozymes A and B in Spodoptera frugiperda Sf21 cells as glutathione-S-transferase fusion proteins via baculovirus infection, isozyme B is folded less efficiently
functional expression of unmodified enzyme and as tagged fusion protein in Spodoptera frugiperda Sf9 cells via baculovirus infection, coexpression of fowl plague virus hemagglutinin HA as endogenous substrate for in vivo activity assay
gene gene TbGT11, DNA and amino acid sequence determination and analysis, gene TbGT11 encodes a Golgi apparatus resident UDP-GlcNAc:alpha3-D-mannoside beta1-2-N-acetylglucosaminyltransferaseI activity (TbGnTI).overexpression of full-length TbGT11 with a C-terminal HA3 epitope tag from a plasmid based on the trypanosome expression vector pLEW82
human beta-1-2-N-acetylglucosaminyltransferase (hGnT1) lacking the first 103 amino acids is expressed as a maltose binding protein (MBP) fusion protein in inclusion bodies in Escherichia coli and refolded using an oxido-shuffling method. Cloning and expression of MBP-hGnT1(D103) and mutant MBP-hGnT1 (D103) enzymes
in one construct, the catalytic domain of human GNT I (abbreviated as NA) is fused to the MNN9 leader (abbreviated as 15) from Saccharomyces cerevisiae to yield construct NA15. In a second construct, a fungal codon-optimized form is combined with the same MNN9 leader to yield construct coNA15. Expression in Aspergillus nidulans and Aspergillus niger
PCR-amplification of CTS region (N-terminal cytoplasmic tail, transmembrane domain, stem region), fusion to GFP (green fluorescent protein), expression of vector in Nicotiana benthamiana mediated by Agrobacterium tumefaciens strain UIA143
the full length GnT-I gene is expressed in Aspergillus oryzae. GnT-1 is functionally expressed and N-acetylglusosamine is transferred to N-glycan of alpha-amylase in vivo
transformation construct contains either the catalytic domain (amino acids 106-447) of wild-type enzyme or D144N rabbit NgTI fused to the CTS region (amino acids 1-102) of Arabidopsis thaliana GnTI under the control of the strong constitutive cauliflower mosaic virus 35S promoter
expression of YFP or CFP fusion enzyme of Arabidopsis and human N-acetylglucosaminyltransferase I, and the chimeric enzyme of Arabidopsis CTS region and human catalytic domain in mutant cgl1-1 Arabidopsis (lacking N-acetylglucosaminyltransferase I), transformed by Agrobacterium tumefaciens
expression of YFP or CFP fusion enzyme of Arabidopsis and human N-acetylglucosaminyltransferase I, and the chimeric enzyme of Arabidopsis CTS region and human catalytic domain in mutant cgl1-1 Arabidopsis (lacking N-acetylglucosaminyltransferase I), transformed by Agrobacterium tumefaciens
2.4.1.101 alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
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