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2.3.1.310: benzoylsuccinyl-CoA thiolase

This is an abbreviated version!
For detailed information about benzoylsuccinyl-CoA thiolase, go to the full flat file.

Reaction

(S)-2-benzoylsuccinyl-CoA
+
CoA
=
benzoyl-CoA
+
succinyl-CoA

Synonyms

(S)-2-benzoylsuccinyl-CoA thiolase, bbsAB

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.310 benzoylsuccinyl-CoA thiolase

Purification

Purification on EC 2.3.1.310 - benzoylsuccinyl-CoA thiolase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
the BbsAB complex is purified in one step via strep-tag affinity chromatography without apparent splitting up into the respective subunits, yielding 50-60 mg pure protein per 10 g wet cell mass, further purified by size exclusion chromatography
Q39VG2; Q39VG1