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2.1.1.273: benzoate O-methyltransferase

This is an abbreviated version!
For detailed information about benzoate O-methyltransferase, go to the full flat file.

Word Map on EC 2.1.1.273

Reaction

S-adenosyl-L-methionine
+
benzoate
=
S-adenosyl-L-homocysteine
 +
methyl benzoate

Synonyms

AlBSMT1, AtBSMT1, BAMT, benzoate-salicylate methyltransferase, benzoic acid carboxyl methyl transferase, benzoic acid carboxyl methyltransferase, benzoic acid carboxymethyl transferase, benzoic acid/SA methyltransferase, benzoic acid/salicylic acid carboxyl methyltransferase, benzoic acid/salicylic acid methyltransferase, BSMT, BSMT1, class II SABATH protein, More, OMT8, PbBSMT methyltransferase, PtSABATH24, S-adenosyl-l-methionine: benzoicacid carboxyl methyl transferase, S-adenosyl-L-methionine:benzoic acid carboxyl methyl transferase, S-adenosyl-L-methionine:benzoic acid carboxyl methyltransferase, S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, SA MTase, SABATH methyltransferase 4, SABATH24, salicylic acid methyltransferase, SAM:benzoic acid carboxyl methyltransferase

ECTree

     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.273 benzoate O-methyltransferase

Cloned

Cloned on EC 2.1.1.273 - benzoate O-methyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
BAMT cDNA expressed in Escherichia coli
-
coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli
expressed with an N-terminal His tag in Escherichia coli
-
gene BAMT, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons
gene BAMT, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, the benzoic acid carboxymethyl transferase from Antirrhinum linkianum appears to be a null allele as mRNA expression is not detected, no alternative splicing
gene BSMT, cloning in Escherichia coli, Arabidopsis thaliana plants are transformed using the floral dip method via Agrobacterium tumefaciens AGL1, recombinant BSMT enzyme expression in Arabidopsis thaliana under the control of a dexamethasone-inducible promoter leading to chlorosis and altered host susceptibility, induced transgenic plants are not able to support large galls and have a brownish appearance of some clubs. The methylester of SA (MeSA) is transported from clubbed Arabidopsis roots to leaves, as shown using heavy isotope-labelled MeSA, and is emitted only from leaves of infected plants, semi- and quantitative RT-PCR expression analysis
gene BSMT, real-time PCR enzyme expression analysis in Plasmodiophora brassicae-infected Brassica oleracea lines
gene BSMT, recombinant expression of 35S::PbBSMT in planta in Arabidopsis thaliana reduces salicylate (SA) levels and increases susceptibility to clubroot, Plasmodiophora brassicae
gene LiBSMT, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis and tree, quantitative real-time PCR expression analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene PbBSMT, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) codon plus
gene SABATH24, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, overview, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression in Escherichia coli strain BL21(DE3)
subcloned into expression vector pET-28a, containing an N-terminal polyhistidine 6-His-tag, and expressed in Escherichia coli