1.5.1.54: methylenetetrahydrofolate reductase (NADH)
This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase (NADH), go to the full flat file.
Reaction
5-methyltetrahydrofolate +
NAD+=
5,10-methylenetetrahydrofolate +
NADH +
H+
Synonyms
5,10-CH2-H4folate reductase, 5,10-methylenetetrahydrofolate reductase, 5,10-methylenetetrahydrofolate reductase (FADH2), 5,10-methylenetetrahydrofolic acid reductase, 5,10-methylenetetrahydropteroylglutamate reductase, 5-methyltetrahydrofolate:(acceptor) oxidoreductase, metF, methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) reductase, methylenetetrahydrofolate reductase, methylenetetrahydrofolic acid reductase, MSMEG_6596, MSMEG_6649, MTHFR1, MTHFR2, N5,10-methylenetetrahydrofolate reductase, N5,N10-methylenetetrahydrofolate reductase
ECTree
Turnover Number
Turnover Number on EC 1.5.1.54 - methylenetetrahydrofolate reductase (NADH)
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0.69 - 4.06
5,10-methylenetetrahydrofolate
0.69
5,10-methylenetetrahydrofolate
pH 7.2, 23°C
4.06
5,10-methylenetetrahydrofolate
pH 7.2, 23°C
0.0002
menadione
mutant E28Q, pH 7.2, 25°C
0.087
menadione
mutant D120N, pH 7.2, 25°C
3.2
menadione
wild-type, pH 7.2, 25°C
0.0002
NADH
mutant E28Q, pH 7.2, 25°C
0.0104
NADH
wild-type, pH 7.2, 25°C
0.057
NADH
mutant D120N, pH 7.2, 25°C
0.2
NADH
mutant E28Q, cosubstrate menadione, pH 7.2, 25°C
0.29
NADH
mutant Q183A, pH 7.2, 4°C
2.2
NADH
wild-type, pH 7.2, 4°C
2.7
NADH
mutant Q183E, pH 7.2, 4°C
23.5
NADH
wild-type, pH 7.2, 15°C
26.67
NADH
mutant A177V, pH 7.2, 15°C
54
NADH
mutant D120N, cosubstrate menadione, pH 7.2, 25°C
55
NADH
wild-type, cosubstrate menadione, pH 7.2, 25°C
1.5.1.54 methylenetetrahydrofolate reductase (NADH)
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