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1.4.1.28: secondary-alkyl amine dehydrogenase [NAD(P)+]

This is an abbreviated version!
For detailed information about secondary-alkyl amine dehydrogenase [NAD(P)+], go to the full flat file.

Reaction

a secondary-alkyl amine
+
H2O
 +
NAD(P)+
=
a ketone
 +
NH3
 +
NAD(P)H
 +
H+

Synonyms

AMDH, AmDH4, amine dehydrogenase, D187_009359, DapB_1, NAD+-dependent amine dehydrogenase, Pmob_1166

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.28 secondary-alkyl amine dehydrogenase [NAD(P)+]

Specific Activity

Specific Activity on EC 1.4.1.28 - secondary-alkyl amine dehydrogenase [NAD(P)+]

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10.1
substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C
104.8
mutant N135V/N163V/R161M/H264L, substrate pentan-2-one, 23°C, pH 9.5
109.6
119.2
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C
120.6
131.6
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C
14.9
substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C
144.5
substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C
159.4
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C
16.4
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C
160.9
substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C
161
substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C
170
-
substrate cyclohexanone, pH not specified in the publication, temperature not specified in the publication
170.1
substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C
175.2
18.8
substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C
196.2
2.7
substrate 3-buten-2-one, cosubstrate NADH, pH 9.5, 23°C
206.5
substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C
233.2
245.6
252.6
257.7
substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C
3 - 8
substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C
3.6
substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C
33.8
substrate 3-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C
34.2
substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C
35
substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C
364
-
substrate isovaleraldehyde, pH not specified in the publication, temperature not specified in the publication
37.1
substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C
388.6
substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C
389
substrate cyclohexanone, cosubstrate NADPH, pH 9.5, 23°C
397.9
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C
4.3
substrate 2-cyclohexen-1-one, cosubstrate NADH, pH 9.5, 23°C
40.1
substrate cyclopentanone, cosubstrate NADH, pH 9.5, 23°C
41.5
substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C
51.9
-
substrate 4-oxopentanoate, pH 7.2, 25°C
60.1
substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C
60.6
substrate cyclopentanone, cosubstrate NADPH, pH 9.5, 23°C
614
substrate cyclohexanone, cosubstrate NADH, pH 9.5, 23°C
614.5
substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C
63.6
substrate 3-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C
7.4
substrate 2-cyclohexen-1-one, cosubstrate NADPH, pH 9.5, 23°C
7.9
substrate 3-buten-2-one, cosubstrate NADPH, pH 9.5, 23°C
73.8
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C
85.2
substrate 4-methylcyclohexanone, cosubstrate NADPH, pH 9.5, 23°C
851.3
substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C
9.3
substrate 4-methylcyclohexanone, cosubstrate NADH, pH 9.5, 23°C
90.4
substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C
955
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C
97.6
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C