1.21.98.3: anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase
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For detailed information about anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase, go to the full flat file.
Reaction
Synonyms
BChE, chlE, Cyan7425_4778, Cyan7822_3999, F_1025, Mg-protoporphyrin IX monomethyl ester cyclase, MPE cyclase, oxygen-independent Mg-protoporphyrin IX monomethyl ester cyclase
ECTree
1.21.98.3 anaerobic magnesium-protoporphyrin IX monomethyl ester cyclaseAdvanced search results
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Cofactor on EC 1.21.98.3 - anaerobic magnesium-protoporphyrin IX monomethyl ester cyclase
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COFACTOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
[4Fe-4S]-center
BchE sequence displays the conserved CXXXCXXC sequence essential for 4Fe-4S cluster formation and an active 4Fe-4S cluster is present in the protein. Isolated protein is yellow-brown in colour with an absorption peak around 410 nm
additional information
membrane-localized BchE requires an additional, heat-sensitive cytosolic cobalamin component for activity, BchE catalysis is not sustained in chimeric experiments when a cytosolic extract from Escherichia coli is used as a substitute
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Cobalamin
vitamin B12-requiring mutants of the bluE and bluB genes of Rhodobacter capsulatus, grown without B12, accumulate Mg-protoporphyrin monomethyl ester and its 3-vinyl-8-ethyl derivative. Cyclase activity in the B12-dependent mutants requires vitamin B12 but not protein synthesis
Cobalamin
dependent on, ligated via Asp248, Glu249, Leu29, Thr71, Val97
S-adenosyl-L-methionine
dependent on, ligated via Phe210, Glu308 and Lys320
