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1.17.9.2: (+)-pinoresinol hydroxylase

This is an abbreviated version!
For detailed information about (+)-pinoresinol hydroxylase, go to the full flat file.

Reaction

(+)-pinoresinol
+ 2 oxidized azurin +
H2O
=
(+)-6-hydroxypinoresinol
+ 2 reduced azurin + 2 H+

Synonyms

BLX42_10690, BLX42_10695, pinAB, pinoresinol alpha-hydroxylase

ECTree

     1 Oxidoreductases
         1.17 Acting on CH or CH2 groups
             1.17.9 With a copper protein as acceptor
                1.17.9.2 (+)-pinoresinol hydroxylase

Systematic Name

Systematic Name on EC 1.17.9.2 - (+)-pinoresinol hydroxylase

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SYSTEMATIC NAME
IUBMB Comments
(+)-pinoresinol:azurin oxidoreductase
Contains FAD. This enzyme, characterized from the bacterium Pseudomonas sp. SG-MS2, catalyses the incorporation of an oxygen atom originating from a water molecule into position C-6 of the lignan (+)-pinoresinol. The enzyme consists of a flavoprotein subunit and a c-type cytochrome subunit. Electrons that originate in the substrate are transferred via the FAD cofactor and the cytochrome subunit to the blue-copper protein azurin.