1.14.14.81: flavanoid 3',5'-hydroxylase
This is an abbreviated version!
For detailed information about flavanoid 3',5'-hydroxylase, go to the full flat file.
Word Map on EC 1.14.14.81
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1.14.14.81
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anthocyanins
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3\'-hydroxylase
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delphinidin
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flavonols
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dihydroflavonols
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4-reductase
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berry
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petunia
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cyanidin
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b-ring
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delphinidin-based
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pelargonidin
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gentian
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leucoanthocyanidins
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3',5'-hydroxylated
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malvidin
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3-o-glucosyltransferase
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dihydrokaempferol
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flavan-3-ols
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cyp75b
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petunidin
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f3\'hs
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co-pigment
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nutrition
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red-skinned
- 1.14.14.81
- anthocyanins
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3\'-hydroxylase
- delphinidin
- flavonols
- dihydroflavonols
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4-reductase
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berry
- petunia
- cyanidin
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b-ring
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delphinidin-based
- pelargonidin
- gentian
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leucoanthocyanidins
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3',5'-hydroxylated
- malvidin
- 3-o-glucosyltransferase
- dihydrokaempferol
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flavan-3-ols
- cyp75b
- petunidin
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f3\'hs
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co-pigment
- nutrition
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red-skinned
Reaction
Synonyms
Cyp75A, CYP75A31, CYP75A42, CYP75A47, CYP75A48, cytochrome P450-like flavonoid 3'5'-hydroxylase, DkF3'5'H, EC 1.14.13.88, F3',5'H, F3'5'H, flavonoid 3',5'-hydroxylase, flavonoid 3'5' hydroxylase, flavonoid 3'5'-hydroxylase, More, plant P450 flavonoid 3',5'-hydroxylase
ECTree
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Engineering
Engineering on EC 1.14.14.81 - flavanoid 3',5'-hydroxylase
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F484Y/S487T
site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme
F48Y
site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme
S487A
site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme
S487Y
site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme
additional information
identification of 202 tea accessions with 120 single nucleotide polymorphisms (SNPs) at F3'5'H locus, genotyping, functional SNP allelic variants within F3'5'H governing the ratio of di/tri-hydroxylated catechins and catechin contents, association mapping and phenotypic variations in the association population
additional information
identification of 202 tea accessions with 120 single nucleotide polymorphisms (SNPs) at F3'5'H locus, genotyping, functional SNP allelic variants within F3'5'H governing the ratio of di/tri-hydroxylated catechins and catechin contents, association mapping and phenotypic variations in the association population
additional information
pollination-constant and non-astringent (PCNA) type mutants lose the ability to produce proanthocyanidin at an early stage in fruit development
additional information
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pollination-constant and non-astringent (PCNA) type mutants lose the ability to produce proanthocyanidin at an early stage in fruit development
additional information
pink-flowered Gentiana scabra plants are bred from spontaneous mutations of Gentiana triflora blue-flowered gentian plants, two different transposable elements, terminal-repeat retrotransposon in miniature, inserted in flavonoid 3,5-hydroxylase gene contribute to pink flower coloration, overview
additional information
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pink-flowered Gentiana scabra plants are bred from spontaneous mutations of Gentiana triflora blue-flowered gentian plants, two different transposable elements, terminal-repeat retrotransposon in miniature, inserted in flavonoid 3,5-hydroxylase gene contribute to pink flower coloration, overview
additional information
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pink-flowered Gentiana scabra plants are bred from spontaneous mutations of Gentiana triflora blue-flowered gentian plants, two different transposable elements, terminal-repeat retrotransposon in miniature, inserted in flavonoid 3,5-hydroxylase gene contribute to pink flower coloration, overview
additional information
introduction of a binary vector to silence flavonoid 3',5'-hydroxylase and anthocyanin 5,3'-aromatic acyltransferase via RNAi silencing downregulating gentiodelphin biosynthesis, thus affecting flower color
additional information
generation of chimeric constructs consisting of cDNA fragments of Osteospermum hybrida F3'5'H and Gerbera hybrida F3'H, EC 1.14.13.21, overview
additional information
redirection of anthocyanin synthesis in Osteospermum hybrida towards pelargonidin derivatives, which are not synthesized in wild-type plants, by expression of Gerbera hybrida dihydroflavonol 4-reductase, the mutant plants show altered flower colours due to synthesis of pelargonidin anthocyaninsssing Gerbera DFR with a construct or RNAi-mediated suppression of F3'5'H activity results in double transgenic plants accumulating predominantly pelargonidin derivatives in flowers, overview
additional information
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recombinant expression of the flavonoid 3',5'-hydroxylase from Petunia sp. in Dendrathemum grandiflora plants leads to competition of the endogenous flavonoid 3'-hydroxylase, EC 1.14.13.21, with the recombinant enzyme for the same substrates in the flavonoid biosynthetic pathway leading to a change in flower petal colors, phenotype, overview
additional information
Viola sp.
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establishment of a bluish color, achieved by delphinidin and derivatives, in Rosa hybrida by overexpression of the F3'5'H gene from Viola sp. and the dihydroflavonol 4-reductase gene from Iris hollandica, while the endogenous dihydroflavonol 4-reductase gene is downregulated, the ability for exclusive accumulation of delphinidin is inherited by the next generations, phenotype, overview
additional information
transgenic Petunia hybrida lines, overexpressing the enzyme from Vitis vinifera, produce high levels of the 3',4',5'-hydroxylated anthocyanin malvidin and very little 3',4'-hydroxylated anthocyanins, they also show a shift from kaempferol to quercetin when compared to the nontransgenic control, flower colours and phenotypes, white and red grape varieties exhibit different temporal expression of F3',5'H1 and cytochrome b5, overview
additional information
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transgenic Petunia hybrida lines, overexpressing the enzyme from Vitis vinifera, produce high levels of the 3',4',5'-hydroxylated anthocyanin malvidin and very little 3',4'-hydroxylated anthocyanins, they also show a shift from kaempferol to quercetin when compared to the nontransgenic control, flower colours and phenotypes, white and red grape varieties exhibit different temporal expression of F3',5'H1 and cytochrome b5, overview
additional information
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transcriptional control of anthocyanin biosynthetic genes in extreme phenotypes for berry pigmentation of naturally occurring grapevines, overview