1.14.14.176: taxadiene 5alpha-hydroxylase

This is an abbreviated version!
For detailed information about taxadiene 5alpha-hydroxylase, go to the full flat file.

Word Map on EC 1.14.14.176

1.14.14.176

taxol

paclitaxel

medicine

taxane

synthesis

bottleneck

oxygenases

nanodiscs

drug development

Reaction

Synonyms

5alphaCYP, CYP725A4, EC 1.14.99.37, oxygenase, taxadiene 5-mono-, taxa-4(5),11(12)-diene 5-hydroxylase, taxa-4(5),11(12)-diene 5alpha-hydroxylase, taxadien-5alpha-hydroxylase, taxadiene 5-hydroxylase, taxadiene 5-monooxygenase, taxadiene synthase, taxadiene-5alpha-hydroxylase

ECTree

1 Oxidoreductases

1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen

1.14.14 With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor

1.14.14.176 taxadiene 5alpha-hydroxylase

Advanced search results

Do not include text mining results

Include

results (more...)

Include

results (more...)

Results	in table
4	Activating Compound
7	AA Sequence
7	Application
1	CAS Registry Number
6	Cloned(Commentary)
3	Cofactor
8	Inhibitors
5	KM Value [mM]
5	Localization
14	Natural Substrates/ Products (Substrates)
14	Organism
6	Pathway
2	pH Optimum
2	Purification (Commentary)
5	Reaction
4	Reaction Type
14	Reference
5	Source Tissue
19	Substrates and Products (Substrate)
19	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Natural Substrates Products

print visible entries

print all entries

Natural Substrates Products on EC 1.14.14.176 - taxadiene 5alpha-hydroxylase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide

Go to Natural Substrate Search

taxa-4(20)-11(12)-diene + AH2 + O2

Taxus sp.

743935

taxa-4(5),11(12)-diene + [reduced NADPH-hemoprotein reductase] + O2

5(12)-oxa-3(11)-cyclotaxane + [oxidized NADPH-hemoprotein reductase] + H2O

Taxus cuspidata

Q6WG30

hydroxylation of the C-5alpha is the first of the oxidations resulting in taxol

687790

OCT is a cyclic ether

taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2

taxa-4(20),11(12)-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O

Taxus sp.

743935

taxa-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2

taxa-4(20),11-dien-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O

7 entries

taxadiene + [reduced NADPH-hemoprotein reductase] + O2

taxadiene-5alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O

Taxus cuspidata

Q6WG30

744544

additional information

Taxus cuspidata

Q6WG30

cDNAs encoding taxa-4(5),11(12)-diene synthase and CYP725A4 are introduced in Nicotiana sylvestris for specific expression in trichome cells. The co-expression does not lead to the production of the expected 5-hydroxytaxa-4(20),11(12)-diene. Instead, taxa-4(5),11(12)-diene is quantitatively converted to a novel taxane

687790