EC Number   |
Protein Variants |
Reference |
|---|
    2.3.3.10 | A110G |
overall reaction rate increases 140fold due to adjustments in the active site that result in additional stabilization of all three steps of the reaction pathway. Crystallization data |
672101 |
| 2.3.3.10 | C117A |
Km-value for acetyl-CoA is 1.7fold higher than the wild-type value, specific activity is 30% of wild-type activity |
660322 |
| 2.3.3.10 | C212C |
mutation causes 6fold decreased maximal velocity |
657850 |
| 2.3.3.10 | F174L |
the mutant shows 10000fold decrease of activity compared to the wild type enzyme |
720239 |
| 2.3.3.10 | G169D |
the mutant is associated with HMGCS2 deficiency |
720239 |
| 2.3.3.10 | G212R |
inactive |
720239 |
| 2.3.3.10 | G388R |
the mutant is associated with HMGCS2 deficiency |
720239 |
| 2.3.3.10 | H188N |
mutation causes 8fold decreased maximal velocity, mutant enzyme does not display substrate inhibition by acetoacetyl-CoA |
657850 |
| 2.3.3.10 | H188N |
the mutant lacks substrate inhibition by acetoacetyl-CoA and shows 8fold decreased enzyme activity |
720647 |
| 2.3.3.10 | H188N/S359A |
10fold increased specific activity, no inhibition by acetoacetyl-CoA |
657850 |
