EC Number   |
Protein Variants |
Reference |
|---|
    2.3.1.41 | A193M |
site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 2.55fold, 12fold reduced catalytic activity |
486936 |
| 2.3.1.41 | C122A |
site-directed mutagenesis, 75% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type |
486938 |
| 2.3.1.41 | C122Q |
site-directed mutagenesis, 500% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type |
486938 |
| 2.3.1.41 | C122S |
site-directed mutagenesis, enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type |
486938 |
| 2.3.1.41 | C161Q |
site-directed mutagenesis, active site Cys mutant, no condensation activity, but 377fold increased decarboxylation activity |
486944 |
| 2.3.1.41 | C163A |
synthase I, site-directed mutagenesis, active site mutant, no activity, but decarboxylates malonyl-[acyl-carrier-protein] |
486943 |
| 2.3.1.41 | C163S |
synthase I, site-directed mutagenesis, active site mutant, increased activity, decarboxylates malonyl-[acyl-carrier-protein] |
486943 |
| 2.3.1.41 | C171Q |
mimics the acyl enzyme intermediate |
718888 |
| 2.3.1.41 | D306A |
synthase I, site-directed mutagenesis, active site mutant, increased activity |
486943 |
| 2.3.1.41 | D66N |
the non-active site mutation alters the structural stability of the mutant protein structures |
-, 753732 |
