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1.3.7.8
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coexpression of benzoyl-CoA reductase with benzoyl-CoA ligase and a putative benzoate transporter in Caldicellulosiruptor bescii strain JWCB018, variable effectiveness of the ribosome binding site sequences utilized in this synthetic operon is observed. The engineered strains do not degrade benzoate. Although the heterologously expressed gene encoding benzoyl-CoA ligase yields a protein that is catalytically active in vitro, expression in Caldicellulosiruptor bescii of six benzoate catabolism-related genes combined in a synthetic operon yields mixed results
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