EC Number   |
Protein Variants |
Reference |
|---|
    1.13.12.7 | A243G |
bioluminescence emission maximum with luciferyl-O-adenosine monophosphate is 599 nm compared to 549 nm for the wild-type enzyme, bioluminescence emission maxima with 5,5-dimethyl-luciferyl-O-adenosine monophosphate are 610 nm and 557 nm compared to 624 nm for the wild-type enzyme |
658085 |
| 1.13.12.7 | A296C/A326C |
specific activity 676% (compared to wild-type 100%), Km (ATP) decreased compared to wild-type, Km (D-luciferin) decreased compared to wild-type, optimal temperature 35° (wild-type 25°C), optimal pH 8.5 (wild-type pH 8), activity remains 54% at 40°C for 5 min (compared to wild-type 0%), t1/2: 40 min at 35°C (compared to wild-type 5 min) |
727630 |
| 1.13.12.7 | A348V |
Km-value for D-luciferin is 8.9fold higher compared to the Km-value of the wild-type enzyme, the turnover-number for D-luciferin is 2fold lower compared to the turnover-number of the wild-type enzyme, the Km-value for MgATP2- is 6.5fold higher compared to the Km-value of the wild-type enzyme |
657942 |
| 1.13.12.7 | AC571TG |
nonsense mutation |
695557 |
| 1.13.12.7 | D436A |
3.4fold increased activity compared to the wild type enzyme |
684400 |
| 1.13.12.7 | D436E |
50% activity compared to the wild type enzyme |
684400 |
| 1.13.12.7 | D436G |
8.5fold increased activity compared to the wild type enzyme |
684400 |
| 1.13.12.7 | D436G |
exhibits 8.5fold higher luciferase activity than the wild type enzyme |
685746 |
| 1.13.12.7 | D436G |
shows 8.5fold increase in luminescence intensity |
685746 |
| 1.13.12.7 | D436N |
50% activity compared to the wild type enzyme |
684400 |
