EC Number   |
Protein Variants |
Reference |
|---|
    1.13.11.33 | A416T |
inactive mutant protein |
660365 |
| 1.13.11.33 | A416V |
inactive mutant protein |
660365 |
| 1.13.11.33 | A417G |
the mutant enzyme converts arachidonic acid to (11R)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid in a 1.5:1 ratio. The wild.type enzyme converts arachidonic acid exlusively (15S)-Hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid. Turnover number for arachidonic acid is 4.6fold lower than that of the wild-type enzyme, no significant change in Km-value |
660365 |
| 1.13.11.33 | D602Y |
15-LOX-2 mutant shows a similar pH-profile as the wild-type enzyme |
702435 |
| 1.13.11.33 | D602Y/V603H |
a strong shift of the pH profile of 15-LOX-2 mutant towards more acidic values |
702435 |
| 1.13.11.33 | F353L |
the ratio of turnover-number to KM-value is 4fold lower than the value for the wild-type enzyme. The mutation strongly alters the positional specificity of the oxygenation of most substrates in favor of 12-lipoxygenation except from (16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid |
658049 |
| 1.13.11.33 | F412E |
mutation slightly impairs membrane binding, mutant enzyme shows 29% of the arachidonic acid oxygenase activity of the wild-type enzyme |
659378 |
| 1.13.11.33 | F70H |
mutation impairs membrane binding, mutant enzyme shows 53% of the arachidonic acid oxygenase activity of the wild-type enzyme |
659378 |
| 1.13.11.33 | H361L |
is devoid of enzymatic activity, can induce phosphorylation of p53 as the wild-type |
704066 |
| 1.13.11.33 | I417A |
naturally occuring mutation, the mutant produces 82% 12-hydroperoxyicosatetraenoate and 18% 15-hydroperoxyicosatetraenoate, in contrast to the wild-type, that produces 14% 12-hydroperoxyicosatetraenoate and 86% 15-hydroperoxyicosatetraenoate |
743691 |
