EC Number   |
Protein Variants |
Reference |
|---|
    1.1.1.86 | D191G |
crystal structure of the mutants (R49E, D83G, D191G and E195S) reveals local conformational changes only in the NADP(H) binding site. Mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme |
762942 |
| 1.1.1.86 | D191K |
mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme |
762942 |
| 1.1.1.86 | D217E |
less than 4% reductoisomerase activity in comparison to wild-type enzyme |
655530 |
| 1.1.1.86 | D217N |
less than 4% reductoisomerase activity in comparison to wild-type enzyme |
655530 |
| 1.1.1.86 | D83G |
crystal structure of the mutants (R49E, D83G, D191G and E195S) reveals local conformational changes only in the NADP(H) binding site. Mutant enzyme exhibits significantly reduced activities compared to wild-type enzyme |
762942 |
| 1.1.1.86 | DELTA1-17 |
mutant with N-terminal 17-residue deletion: cellular localisation similar to wild-type, introduction into an ilv5DELTA strain, lacking the ilv5 gene, complements isoleucine and valine synthesis |
684636 |
| 1.1.1.86 | DELTA1-33 |
mutant with N-terminal 33-residue deletion: cellular localisation predominatly in the cytosol rather than in mitochondria. Deletion of the N-terminal 33 residues is sufficient to largely impair the protein's targeting to mitochondria. Introduction into an ilv5DELTA strain, lacking the ilv5 gene, complements isoleucine and valine synthesis |
684636 |
| 1.1.1.86 | DELTA1-40 |
mutant with N-terminal 40-residue deletion: cellular localisation throughout the cytosol rather than in mitochondria |
684636 |
| 1.1.1.86 | DELTA1-46 |
mutant with N-terminal 46-residue deletion: cellular localisation throughout the cytosol rather than in mitochondria. This mutant shows the largest steady-state protein level. Introduction into an ilv5DELTA strain, lacking the ilv5 gene, does not complements isoleucine and valine synthesis. Introduction into an industrial lager brewing strain, a robust expression of DELTA46 is as effective as that of a wild-type Ilv5p in lowering the total Vicinal diketones production in a 2l scale fermentation trial. Additional expression of DELTA46 does not alter the quality of the resultant beer in terms of contents of aromatic compounds and organic acids |
684636 |
| 1.1.1.86 | DELTA1-53 |
mutant with N-terminal 53-residue deletion: cellular localisation throughout the cytosol rather than in mitochondria |
684636 |
