EC Number |
IC50 Value |
IC50 Value Maximum |
Inhibitor |
Reference |
---|
2.3.3.21 | 0.041 |
- |
isoleucine |
mutant V468A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
2.3.3.21 | 0.041 |
- |
isoleucine |
V468A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.086 |
- |
isoleucine |
wild-type, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.086 |
- |
isoleucine |
wild-type, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
2.3.3.21 | 0.153 |
- |
isoleucine |
H400A/H408A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.153 |
- |
isoleucine |
mutant H400A/H408A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
2.3.3.21 | 0.177 |
- |
isoleucine |
mutant T464A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of isoleucine (approx. 0-10 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
2.3.3.21 | 0.177 |
- |
isoleucine |
T464A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.343 |
- |
leucine |
mutant V468A, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.343 |
- |
leucine |
mutant V468A, in the presence of 0.6 mM pyruvate, 4 mM acetyl-CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS and a varied concentration of leucine (approx. 0-20 mM) in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |