2.4.1.182 | purified soluble recombinant enzymes, LpxB in the apo form and bound to UDP: LpxB7S, SeMet-LpxB7S, LpxB7S with UDP, and mutant LpxB6S (V66S/V68S/L69S/L72S/L75S/L76S), the ligand-bound structure of LpxB is obtained by soaking crystals with 10mM UDP-N-acytlglucosamine (UDP-GlcNAc), X-ray diffraction structure determination and analysis at 1.98-3.43 A resolution |