EC Number |
---|
2.1.1.85 | enzyme in complex with an actin peptide (residues 66-80) that contains His73 in the presence of S-adenosyl-L-homocysteine, hanging drop vapor diffusion method, using either 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000, or 0.2 M ammonium sulfate or 0.2 M sodium chloride, 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350 and 0.1 M Bis-Tris pH 5.5, 25% (w/v) polyethylene glycol 3350 |
2.1.1.85 | S-adenosyl-L-homocysteine-bound enzyme in complex with either an unmodified beta-actin peptide or its His-methylated variant, sitting drop vapor diffusion method, using 0.1 M sodium cacodylate trihydrate (pH 6.5), 0.2 M magnesium acetate tetrahydrate, and 20% (w/v) polyethylene glycol 8000 |
2.1.1.85 | sitting drop vapor diffusion method, using 0.2 M ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.6 and 30% (w/v) polyethylene glycol 4000 |