  1.14.14.B14 | analysis of the PqsL structure (PDB ID 2X3N), which has been solved to a resolution of 1.75 A and remodeling of a loop region, which aligns with a loop reported to be involved in NADPH and FAD binding in pHBH, comparison to the structure of pHBH of Pseudomonas fluorescens (PDB code 1PBE), structure-function analysis, detailed overview. The conformation found in PqsL is closer to the open state, which is rather unusual for a group A monooxygenase without substrate bound. The enzyme is not capable of binding NAD(P)H in the alleged mode because several of the crucial positions are replaced by dysfunctional substitutions |
