EC Number   |
|---|
    1.1.1.86 | - |
| 1.1.1.86 | ammonium sulfate precipitation |
| 1.1.1.86 | ammonium sulfate precipitation, crystal structure of the enzyme complexed with NADPH, two magnesium ions and N-hydroxy-N-isopropyloxamate, a herbicidal transition state analog determined at 1.65 A resolution, recombinant enzyme overexpressed in Escherichia coli |
| 1.1.1.86 | crystal structure at a 1.75 A resolution |
| 1.1.1.86 | crystal structures of the enzyme in complex with two transition state analogues, cyclopropane-1,1-dicarboxylate and N-isopropyloxalyl hydroxamate. Hanging-drop vapor-diffusion method |
| 1.1.1.86 | crystallized with 2-aceto-2-hydroxybutanoate, Mn2+ and NADPH |
| 1.1.1.86 | enzyme KARI in complex with Mg2+ or with Mg2+ and NADPH, hanging-drop method by vaporphase diffusion at 18°C, 0.003 ml of protein solution containing 6 mg/ml enzyme, 50 mM Hepes, pH 7.5, 5 mM NADPH, and 5 mM MgCl2, is mixed with 0.001 ml of reservoir solution containing 0.2 M magnesium chloride hexahydrate, 0.1 M Tris-HCl, pH 8.5, and 15% w/v PEG 4000, a few days to 3 months, X-ray diffraction structure determination and analysis at 1.55 A and 2.80 A resolution, respectively, molecular replacement |
| 1.1.1.86 | hanging drop method |
| 1.1.1.86 | hanging drop method, cocrystallization of 3-((methylsulfonyl)methyl)-2H-benzo[b][1,4]oxazin-2-one in complex with the enzyme |
| 1.1.1.86 | hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 5.0, 20% (w/v) w/v polyethylene glycol 1500 |
