EC Number |
Ki Value [mM] |
Ki Value maximum [mM] |
Inhibitor |
Reference |
---|
2.3.3.21 | 0.009 |
- |
isoleucine |
mutant V468A, calculated from the Cheng-Prusoff equation |
702132 |
2.3.3.21 | 0.009 |
- |
isoleucine |
V468A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.019 |
- |
isoleucine |
wild-type, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.019 |
- |
isoleucine |
wild-type, calculated from the Cheng-Prusoff equation |
702132 |
2.3.3.21 | 0.024 |
- |
isoleucine |
mutant T464A, calculated from the Cheng-Prusoff equation |
702132 |
2.3.3.21 | 0.024 |
- |
isoleucine |
T464A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.043 |
- |
isoleucine |
H400A/H408A 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.043 |
- |
isoleucine |
mutant H400A/H408A, calculated from the Cheng-Prusoff equation |
702132 |
2.3.3.21 | 0.075 |
- |
leucine |
mutant V468A, 0.6 mM pyruvate, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, variable inhibitor concentrations, 0.1 M HEPES, pH 7.7 |
702132 |
2.3.3.21 | 0.075 |
- |
leucine |
mutant V468A, calculated from the Cheng-Prusoff equation |
702132 |