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EC Number	pH Minimum	pH Maximum	Commentary	Reference
1.13.12.7	-999	-	buffers with pKa from 7.2 to 8.4 tested and no relationship with activity found	484843
1.13.12.7	2.5	10.5	pH activity range, recombinant circular enzyme	746213
1.13.12.7	4	10	pH activity range, recombinant wild-type enzyme	746213
1.13.12.7	6	7.8	bioluminescence spectra independent from pH, for mutants Y35N, Y35H and F16L/A40S	696164
1.13.12.7	6.5	8.5	pH 6.5: about 45% of activity maximum, pH 8.5: about 35% of activity maximum	484843
1.13.12.7	6.5	9	-	672284
1.13.12.7	6.5	9.6	-	700622
1.13.12.7	7	8	-	484833
1.13.12.7	7	9	pH 7.0: about 80% of maximal activity, pH 9.0: about 70% of maximal activity, isoenzyme AL1	765525
1.13.12.7	7.5	8	pH below 7.5 emission shifts to longer wavelengths	484831

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Release 2023.1 (January 2023)