EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.4.3.2 | -999 |
- |
freezing causes reversible inactivation |
391815 |
| 1.4.3.2 | -999 |
- |
labile to heating at 60°C |
391791 |
| 1.4.3.2 | -999 |
- |
reduced form of the enzyme is much more stable to heat than oxidized form, substrates: e.g. L-Leu, L-Phe, L-Met protect from heat denaturation, no protection by D-leucine and L-lysine |
391774 |
| 1.4.3.2 | -70 |
- |
the enzyme is stable at low temperatures (-20°C, -70°C) for one week and completely loses its activity after heating at 70°C for 10 min |
691918 |
| 1.4.3.2 | 0 |
50 |
stable within this range |
670963 |
| 1.4.3.2 | 4 |
- |
3 months, no loss of activity |
391772 |
| 1.4.3.2 | 4 |
- |
at temperatures lower than 4°C, enzymatic activity is completely lost |
692650 |
| 1.4.3.2 | 20 |
60 |
the enzyme has half-life values of 21.2, 8.3, 3.6, 3.1, 2.6 h at 30, 35, 40, 50, 60°C, respectively, and melting temperature of 61.3°C |
743253 |
| 1.4.3.2 | 25 |
- |
1 month, no loss of activity |
391772 |
| 1.4.3.2 | 25 |
50 |
inactivation in 50 mM triethylamine/HCl buffer, pH 7.6 in the same range as 50 mM potassium-phosphate buffer pH 6.6 |
651026 |
