EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.2.1.12 | -999 |
- |
incubation at 70-85°C results in an apparent activation |
287906 |
| 1.2.1.12 | -999 |
- |
temperature of 50% inactivation increases by 15°C, when KCl concentration is increased from 1 M to 4 M or when 1 M KCl is replaced by 1 M (NH4)2SO4 |
287907 |
| 1.2.1.12 | -999 |
- |
the addition of either NAD+ or glyceraldehyde 3-phosphate shifts this equilibrium towards a compact dimer, that is more stable than the unliganded one, thermal scanning fluorimetry |
741965 |
| 1.2.1.12 | -999 |
- |
the thermal stability of glyceraldehyde-3-phosphate dehydrogenase increases significantly in the presence of its cofactor NAD+. Incubation of Acropora millepora GAPDH with NAD+ increases its melting temperature by 15°C |
763684 |
| 1.2.1.12 | -999 |
- |
three salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer |
287962 |
| 1.2.1.12 | 15 |
- |
5 min, stable |
287919 |
| 1.2.1.12 | 15 |
- |
activity is lost after several min below 15°C |
726827 |
| 1.2.1.12 | 15 |
60 |
pre-incubation for 10 min at temperatures varying between 15°C and 30°C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35°C and results in total activity loss at 60°C |
684121 |
| 1.2.1.12 | 15 |
70 |
pre-incubation for 10 min at temperatures varying between 20 and 32°C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35°C and results in total activity loss at 70°C |
684127 |
| 1.2.1.12 | 22 |
- |
at room temperature the enzyme is stable for several weeks |
726827 |
