EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.14.18.1 | -999 |
- |
enzyme is relatively stable at 60°C, with 55% loss of activity, unstable at temperatures above 75°C. The enzyme is incubated at different temperatures (45-85°C) for 30 min and, after cooling, the residual enzyme activity is measured |
686847 |
| 1.14.18.1 | -999 |
- |
from thermostability studies, the Ki value increases with temperature and the results suggest that the enzyme is unstable above 45°C. Above 45°C, the PPO activity decreases with increasing temperature. Strong evidence that the denaturalization temperature of PPO is near 70°C |
687243 |
| 1.14.18.1 | -999 |
- |
PPO1 appears to be more stable at high temperatures than PPO2 or PPO3 |
687250 |
| 1.14.18.1 | -999 |
- |
results from kinetic and thermodynamic parameters for the thermal inactivation evidence that the partially purified PPO is a biocatalyst, whose inactivation process is related to aggregation of partially unfolded enzyme molecules |
745557 |
| 1.14.18.1 | -999 |
- |
the thermal inactivation follows a first-order kinetic model, with activation energy of Ea150.46 kJ/mol |
746341 |
| 1.14.18.1 | -999 |
- |
thermal inactivation of PPO is investigated at 35, 55 and 75°C. Results of the heat inactivation studies suggest that PPO belongs to the group of heat-stable enzymes |
686484 |
| 1.14.18.1 | 5 |
25 |
pH 6.0, 1 h, cuticle-bound enzyme is stable |
636341 |
| 1.14.18.1 | 10 |
20 |
purified native enzyme, 60 min, over 90% activity remaining |
745474 |
| 1.14.18.1 | 10 |
70 |
the activity of recombinant PPO gradually increases with temperature ranging from 10 to 45°C, but followed by a decrease at higher temperatures, the residual soluble PPO activity remains above 60% after 40 min at 30, 40 and 50°C and then decreases significantly when temperature reaches at 60°C, with only 55% activity in just 10 min. When the temperature is raised to 80°C, the enzyme retains less than 18% of its original activity after 20 min and even less after prolonged incubation. |
698038 |
| 1.14.18.1 | 10 |
80 |
the enzyme is stable between 10 and 30°C after 24 h incubation, The enzyme activity declines as the temperature increases but the enzyme is not completely inactivated even at 80°C |
712053 |
