EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.1.86 | -999 |
- |
deletion mutant DELTA423-4301/F431S shows significantly lower temperature stability than wild type enzyme |
639185 |
1.1.1.86 | 25 |
85 |
stable, based on CD analysis |
763188 |
1.1.1.86 | 40 |
- |
5 min, about 90% loss of activity at pH 6.2, about 55% loss of activity at pH 6.5, 35% loss of activity oH 7.0, 30% loss of activity at pH 7.5, about 85% loss of activity at pH 8.0, about 90% loss of activity at pH 8.5 |
639171 |
1.1.1.86 | 50 |
- |
at 50°C, the enzyme shows an half-life of 71 h |
737785 |
1.1.1.86 | 86 |
- |
Tm-value |
763730 |
1.1.1.86 | 95 |
- |
at 95°C, the enzyme is completely unfolded, and it does not refold even when it is slowly cooled to 25°C. Presumably the unfolding process is irreversible, especially after dissociation of the intertwined knot domains |
763730 |
1.1.1.86 | 95 |
- |
the enzyme is highly thermostable with half-maximal residual activity at 95°C |
731279 |