EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   1.1.1.50 | 40 |
- |
15 min stable in presence of mercaptoethanol, 10% loss of activity in absence of mercaptoethanol |
389522 |
| 1.1.1.50 | 40 |
- |
purified recombinant His-tagged enzyme, stable up to |
741361 |
| 1.1.1.50 | 45 |
- |
heating for 10 min, rapid heat denaturation over 50°C |
389548 |
| 1.1.1.50 | 47.2 |
- |
melting temperature, mutant D249S |
695886 |
| 1.1.1.50 | 47.9 |
- |
melting temperature, mutant D249A |
695886 |
| 1.1.1.50 | 49.41 |
- |
melting temperature, mutant D249K |
695886 |
| 1.1.1.50 | 50 |
- |
30-60 min stable |
347743 |
| 1.1.1.50 | 50 |
- |
denaturation above |
389531 |
| 1.1.1.50 | 54 |
- |
the melting temperature of the wild type enzyme is at 54.76°C |
695886 |
| 1.1.1.50 | 54.8 |
- |
melting temperature, wild-type |
695886 |
