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EC Number	Temperature Optimum [�C]	Temperature Optimum Maximum [�C]	Commentary	Reference
1.13.12.7	-999	-	shift in bioluminescence spectra to red region by increase in temperature to 42°C	696164
1.13.12.7	-999	-	shift of emission to red emitting light	698393
1.13.12.7	10	-	mutant enzyme H461D	764010
1.13.12.7	10	-	the activity of Pyrocoelia sp. RF-2016 at 10°C is about 1.3times stronger than the Pyrocoelia pyralis luciferase activity at 25°C	765518
1.13.12.7	20	-	assay at	727160
1.13.12.7	20	-	in the presence of [1,1,3,3-tetramethylguanidine][trichloroacetate] and [1,1,3,3-tetramethylguanidine][triflouroacetate], enzyme activity reaches to maximum at 20 °C	726696
1.13.12.7	20	-	mutant enzyme H489M	764010
1.13.12.7	22	-	-	745975
1.13.12.7	22	-	assay at	765524
1.13.12.7	22	-	assay at room temperature	744408

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Release 2023.1 (January 2023)