EC Number   |
|---|
   2.1.1.224 | - |
| 2.1.1.224 | a plasmid-borne cfr geneis transfromed into a uL3-depleted Escherichia coli strain containing either wild-type L3 or L3 with one of seven mutations, G147R, Q148F, N149S, N149D, N149R, Q150L, or T151P, expressed from plasmid-carried rplC genes. The L3 mutations are well tolerated, with small to moderate growth rate decreases. The presence of Cfr has a very minor influence on the growth rate. The transformants show resistance to linezolid, tiamulin, florfenicol, and Synercid (a combination of quinupristin and dalfopristin [Q-D]) |
| 2.1.1.224 | expressed as C-terminally His6-tagged enzyme, wild-type and mutant enzymes C125A, C129A and C132A |
| 2.1.1.224 | expressed in Escherichia coli Rosetta2(DE3)pLysS cells |
| 2.1.1.224 | gene cfr(C), DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis, cfr(C) is constitutively transcribed, gene cfr(C) is part of a putative 24 kb-transposon, which generates a detectable circular intermediate |
| 2.1.1.224 | gene cfr(C), DNA and amino acid sequence determination and analysis, the cfr-like is cloned in Clostridium difficile strain 630DELTAerm in which it confers resistance to phenicols, lincosamides, oxazolidinones, pleuromutilins, and streptogramin A (PhLOPSA) |
| 2.1.1.224 | gene cfr(E), DNA and amino acid sequence determination and analysis, genotyping, sequence comparisons and phylogenetic analysis of different cfr gene variants, recombinant expression of tagged enzyme Cfr(E) in Escherichia coli strain Rosetta(DE3)pLysS |
| 2.1.1.224 | gene cfr, operon isc, co-expression with isc proteins, expression of His6-tagged Cfr under control of an arabinose-inducible promoter in Escherichia coli strain BL21Star |
| 2.1.1.224 | gene cfr, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain +Rosetta2(DE3) |
| 2.1.1.224 | gene cfr, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain JW2501-1 (rlmN knockout) and AS19 |
