EC Number   |
|---|
   1.2.1.30 | - |
| 1.2.1.30 | DNA and amino acid sequence determination and analysis, phylogenetic analysis |
| 1.2.1.30 | DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli resulting in decreased activity due to a lack of post-translational phosphopantetheinylation of a serine group in the recombinant CAR that is necessary for activity. Coexpression with the recombinant phosphopantetheinyl transferase (PPTase) from Bacillus subtilis (Sfp) or cell-free extracts from Nocardia iowensis containing native PPTase converted niCAR to its holoenzyme state, increases the enzyme activity 20fold. Recombinant enzyme expression in Saccharomyces cerevisiae and Saccharomyces pombe and an engineered aldehyde-accumulating Escherichia coli strain for de novo production of vanillin from glucose |
| 1.2.1.30 | expressed in Escherichia coli BL21(DE3) cells |
| 1.2.1.30 | plasmids pHAT305(Placcar), pPV2.83(Placcar Placnpt), pPV2.84(Placcar Placgdh) or pPV2.85(Placcar Plac npt gdh) expressed in Escherichia coli BL21-CodonPlus(DE3)-RP cells |
| 1.2.1.30 | recombinant car expressed in Escherichia coli BL21-CodonPlus(DE3)-RP/pHAT305 and in Escherichia coli BL21-CodonPlus(DE3)-RP/pPV1.184 cells |
| 1.2.1.30 | recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain MG1655 |
| 1.2.1.30 | sequence comparisons and phylogenetic analysis, recombinant coexpression of His-tagged enzyme in Escherichia coli |
| 1.2.1.30 | sequence comparisons and phylogenetic analysis, recombinant coexpression of His-tagged enzyme with Escherichia coli phosphopantetheinyl transferase, EcPPTase, in Escherichia coli strain MG1655 RARE |
