EC Number   |
|---|
    1.14.18.1 | - |
| 1.14.18.1 | Agaricus bisporus possesses genes coding for six different tyrosinases (AbPPO1-AbPPO6), full-length gene AbPPO4 (1-565) DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally GST-tagged tyrosinase 4 from gene AbPPO4 containing 23 mutations in Escherichia coli as latent enzyme |
| 1.14.18.1 | cloning of cDNA |
| 1.14.18.1 | DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha |
| 1.14.18.1 | DNA and amino acid sequence determination and analysis, sequence comparisons |
| 1.14.18.1 | Escherichia coli CC118gammapir with plasmid mini-Tn5 derivative pUT-Km1 (transposon donor to introduce mutagenisis), Escherichia coli HB101 with plasmid pRK600 (helper), and Escherichia coli TOP 10 as general cloning host for triparental mating experiments with Pseudomonas putida as recipient |
| 1.14.18.1 | expressed in Escherichia coli BL21 cells |
| 1.14.18.1 | expressed in Escherichia coli BL21(DE3) cells |
| 1.14.18.1 | expressed in HEK-293T cells |
| 1.14.18.1 | expressed in Pichia pastoris |
