EC Number |
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1.1.1.14 | - |
1.1.1.14 | DNA and amino acid sequence determination and analysis, co-expression with gene sldB in Escherichia coli strain JM109, sldB and PQQ are required for recombinant activity in Escherichia coli, co-expression of sldB is also required for enzyme activity in vivo |
1.1.1.14 | electrophoretic karyotyping and array-based comparative genomic hybridization (array-CGH), comparison of four different species derived from the Saccharomyces sensu stricto complex of 22 distillery strains, overview. The genomic diversity is mainly revealed within subtelomeric regions and the losses and/or gains of fragments of chromosomes I, III, VI and IX are the most frequently observed. Statistically significant differences in the gene copy number are documented in six functional gene categories: 1. telomere maintenance via recombination, DNA helicase activity or DNA binding, 2. maltose metabolism process, glucose transmembrane transporter activity, 3. asparagine catabolism, cellular response to nitrogen starvation, localized in cell wall-bounded periplasmic space, 4. siderophore transport, 5. response to copper ion, cadmium ion binding and 6. L-iditol 2-dehydrogenase activity. Distillery yeasts are diploid. Gene ontology overrepresentation profiles are species-specific |
1.1.1.14 | expressed in Pseudomonas putida strain IFO3738 fused to 6 x His-tag |
1.1.1.14 | expression in Escherichia coli |
1.1.1.14 | expression in Escherichia coli strain BL21 |
1.1.1.14 | expression of wild-type and mutant enzymes in Escherichia coli |
1.1.1.14 | gene GoSCR, recombinant expression of His-tagged enzyme GoSCR in Escherichia coli strain BL21 (DE3), coexpression with 2,3-butanediol dehydrogenase (BDHA) from Bacillus subtilis for asymmetric reduction of 2-hydroxyacetophenone (2-HAP) to (R)-1-phenyl-1,2-ethanediol ((R)-PED) or (S)-1-phenyl-1,2-ethanediol ((S)-PED) |
1.1.1.14 | gene MdSDH5, DNA and amino acid sequence determination and analysis, transient expression of MdSDH5-GFP and MdSDH6-GFP in the mesophyll protoplast of Arabidopsis thaliana |
1.1.1.14 | gene MdSDH6, DNA and amino acid sequence determination and analysis, phylogenetic tree, transient expression of MdSDH5-GFP and MdSDH6-GFP in the mesophyll protoplast of Arabidopsis thaliana |