EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.15.28 | (25R)-25-bromo-27-norcholesterol + 6 reduced [2Fe-2S] ferredoxin + 3 O2 |
- |
Mycobacterium tuberculosis |
(25R)-25-bromo-27-norcholesterol-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |
1.14.15.28 | (25R)-25-bromo-27-norcholesterol + 6 reduced [2Fe-2S] ferredoxin + 3 O2 |
- |
Mycobacterium tuberculosis H37Rv |
(25R)-25-bromo-27-norcholesterol-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |
1.14.15.28 | (25R)-26-hydroxycholest-4-en-3-one + reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
- |
Mycobacterium tuberculosis |
(25R)-26-oxocholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.28 | (25R)-26-oxocholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 |
- |
Mycobacterium tuberculosis |
(25R)-3-oxocholest-4-en-26-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O |
- |
? |
1.14.15.28 | 26-methylcholesterol + 6 reduced [2Fe-2S] ferredoxin + 3 O2 |
- |
Mycobacterium tuberculosis |
(25R)-3beta-hydroxycholest-5-en-27-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |
1.14.15.28 | 26-methylcholesterol + 6 reduced [2Fe-2S] ferredoxin + 3 O2 |
- |
Mycobacterium tuberculosis H37Rv |
(25R)-3beta-hydroxycholest-5-en-27-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |
1.14.15.28 | cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin |
- |
Mycolicibacterium smegmatis |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |
1.14.15.28 | cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin |
- |
Mycolicibacterium smegmatis mc(2)155 |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |
1.14.15.28 | cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin + 3 O2 |
the enzyme can receive electrons from ferredoxin reductase in vitro, its natural electron donor is not known yet. The enzyme of the bacterial pathogen is involved degradation of the host cholesterol |
Mycobacterium tuberculosis |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |
1.14.15.28 | cholest-4-en-3-one + 6 reduced [2Fe-2S] ferredoxin + 3 O2 |
catalyses the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol. The products are exclusively in the (25R) conformation |
Mycobacterium tuberculosis |
(25R)-3-oxocholest-4-en-26-oate + 6 oxidized [2Fe-2S] ferredoxin + 4 H2O |
- |
? |