EC Number   |
Storage Stability |
|---|
    2.3.3.10 | -20°, stored in presence of dithiothreitol and 30-50% glycerol purified enzyme retains complete activity for up to 3 months |
| 2.3.3.10 | -20°C, may be stored in 30% glycerin solution for 2-3 months with no loss of activity |
| 2.3.3.10 | -20°C, purified synthase may be stored for 2-3 months with little loss of activity by adding 30% glyerol |
| 2.3.3.10 | -20°C, stored in presence of 30% glycerol and 5 mM dithiothreitol, remains active for several months |
| 2.3.3.10 | -80°C, recombinant enzyme, negligible loss of activity after 1 year |
| 2.3.3.10 | -85°C, in presence of 30% glycerol stable for over 1 year |
| 2.3.3.10 | -90°C, purified synthases II-IV in 20 mM potassium phosphate, pH 7.0, 5 mM dithiothreitol, can be stored as pellets following precipitation by 60% saturated ammonium sulfate up to 3 months without loss of activity |
| 2.3.3.10 | 0°C, purified synthase I stored frozen in 40% glycerol containing 20 mM potassium phosphate, pH 7.0, 5 mM dithiothreitol, stable for 3-4 months |
| 2.3.3.10 | 4°C, enzyme loses activity in absence of glycerol |
| 2.3.3.10 | 4°C, in presence of 30% glycerol stable for several days |
