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EC Number Renatured (Commentary) Reference
Show all pathways known for 1.14.18.1Display the word mapDisplay the reaction diagram Show all sequences 1.14.18.1all proteins including hTyrCtr and OCA1B-related mutant variants are a subjected to equilibrium unfolding/refolding using 0.001 mM protein in 10 mM phosphate buffer, pH 7.4, 5 h at 8 M urea, followed by dilution with 10 mM phosphate buffer, pH 7.4 to 0-8 M urea and incubated for 24 h at room temperature 744758
Show all pathways known for 1.14.18.1Display the word mapDisplay the reaction diagram Show all sequences 1.14.18.1recombinant enzyme from inclusion bodies, extensive standardization of buffers and methods of refolding such as 1. dialysis, 2. on-column refolding, and 3. rapid dilution yield active PPO from solubilized inclusion bodies with copper content of 0.880 atom/molecule of protein, method development, detailed overview 746343
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