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EC Number Renatured (Commentary) Reference
Show all pathways known for 1.13.12.7Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.7in the presence of the DnaKJE chaperone system thermally inactivated monomeric bacterial luciferase refolds. Monomeric bacterial luciferase thermally inactivated in the presence of ATPindependent trigger factor is not able to refold 744279
Show all pathways known for 1.13.12.7Display the word mapDisplay the reaction diagram Show all sequences 1.13.12.7renaturation is initiated by diluting the denaturant in 50 mM HEPES-KOH, pH 7.5, 10 mM Mg(OAc)2, 70 mM KOAc, 50 mM imidazole, and 1 mM dithiothreitol without urea, refolding of firefly luciferase from a denatured state is a slow process, its rate and productivity depend on molecular chaperones of the Hsp70 family, Hsp70-dependent refolding restores 55% of the initial enzymatic activity, immobilization leads to a higher refolding yield owing to the prevention of intermolecular aggregation 688898
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