EC Number   |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Reference |
|---|
    2.3.3.21 | 0.74 |
- |
acetyl-CoA |
mutant Y454A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
| 2.3.3.21 | 0.8 |
- |
acetyl-CoA |
mutant I458A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
| 2.3.3.21 | 1.4 |
- |
acetyl-CoA |
L451V 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 |
702132 |
| 2.3.3.21 | 1.4 |
- |
acetyl-CoA |
mutant L451V, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
| 2.3.3.21 | 1.5 |
- |
pyruvate |
wild-type, 4 mM acetyl CoA, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 |
702132 |
| 2.3.3.21 | 2.6 |
- |
acetyl-CoA |
wild-type, 60 microM isoleucine, 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 |
702132 |
| 2.3.3.21 | 2.6 |
- |
acetyl-CoA |
wild-type, in the presence of 0.06 mM isoleucine, 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
| 2.3.3.21 | 3.2 |
- |
acetyl-CoA |
D431A 0.6 mM pyruvate, 0.8 mM MnCl2, 50 mM KCl, 15 nM enzyme, 0.1 M HEPES, pH 7.7 |
702132 |
| 2.3.3.21 | 3.2 |
- |
acetyl-CoA |
mutant D431A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
| 2.3.3.21 | 3.8 |
- |
acetyl-CoA |
mutant T464A, in the presence of 0.8 mM MnCl2, 50 mM KCl, 15 nM CMS, in a total volume of 50 microL using 0.1 M Hepes, pH 7.7 |
702132 |
