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EC Number General Information Commentary Reference
Display the reaction diagram Show all sequences 1.5.99.14physiological function 6-hydroxypseudooxynicotine dehydrogenase utilizes the electron transfer flavoprotein EtfAB as the physiological electron acceptor. The electrons are further transferred from the reduced EtfAB to coenzyme Q by the catalysis of electron transfer flavoprotein:ubiquinone oxidoreductase. Disruption of the EtfAB genes in the nicotine-degrading gene cluster decreases the growth rate of Agrobacter tumefaciens S33 on nicotine but not on 6-hydroxy-3-succinoylpyridine -, 763956
Display the reaction diagram Show all sequences 1.5.99.14physiological function Rid protein (participating in metabolite damage repair) encoded by a gene in the nicotine-degrading gene cluster enhances the reaction of 6-hydroxypseudooxynicotine dehydrogenase. Rid relieves the toxicity of the presumed imine intermediate produced in the 6-hydroxypseudooxynicotine dehydrogenase reaction and, in the presence of Rid, 6-hydroxypseudooxynicotine dehydrogenase maintains a high level of activity and the amount of the reaction product is increased by at least 5fold. Disruption of the Rid gene leads to slower growth on nicotine -, 763981
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