EC Number   |
General Information |
Reference |
|---|
    1.13.12.7 | evolution |
two paralogous firefly luciferase genes, Luc1 and Luc2. Both gene products have luciferase activity to emit green light. Only Luc1 exhibits spectral pH-sensitivity. Phylogenetic analysis suggests that the pH-sensitivity is developed during the course of the evolution of Luc1 lineage |
765522 |
| 1.13.12.7 | malfunction |
the single conservative amino acid change tyrosine to phenylalanine at position 255 accounts for the entire emission color difference |
745975 |
| 1.13.12.7 | more |
usage of three different methods, molecular dynamics (MD) simulation, B-FITTER and framework rigidity optimized dynamics algorithm (FRODA) to determine the flexible regions of Photinus pyralis luciferase: fragment 197-207, fragment 471-481, and fragment 487-495. Introduction of proline within most flexible regions is used to rigidify these flexible regions resulting in mutants D476P and H489P. Analysis of the relationship between stability, activity and flexibility of wild-type and mutant enzymes, molecular dynamics simulations, modelling, overview |
744408 |
| 1.13.12.7 | physiological function |
beetle bioluminescence is the result of two half-reactions catalyzed by a luciferase (Luc) in which the substrate D-firefly luciferin is converted into the corresponding adenylate D-firefly luciferin-AMP, followed by a multistep oxidative process that produces light via a single electron transfer process. Among the Lampyridae, one of the three families of bioluminescent beetles, light emission typically ranges from green to yellow-green in color having emission maxima (lambdamax) of about 548-568 nm |
745975 |
| 1.13.12.7 | physiological function |
catalyzes visible light emission |
698393 |
| 1.13.12.7 | physiological function |
fatty acyl-CoA synthesis, acyl-adenylate/thioester-forming enzyme |
697221 |
| 1.13.12.7 | physiological function |
firefly luciferase from North American Photinus pyralis is a monooxygenase that performs ATP-dependent conversion of luciferin into a luciferyl-adenylate, which is oxidized in a multistep reaction to electronically excited oxyluciferin |
744062 |
| 1.13.12.7 | physiological function |
luciferase is the key component of light production in bioluminescence process |
765646 |
