EC Number |
---|
2.4.1.18 | - |
2.4.1.18 | 2 isozymes |
2.4.1.18 | 25.4fold after immobilized metal-ion affinity chromatography |
2.4.1.18 | 3.45fold after Ni-NTA affinity chromatography |
2.4.1.18 | 4 isozymes |
2.4.1.18 | about 19.0 to 101.9fold after Sephacryl S-200 gel filtration and Q2-sepharose anion exchange chromatography |
2.4.1.18 | ammonium sulfate, Q-Sepharose column, ultrafiltration, gel filtration with Superdex 200 column, native polyacrylamide gel elektrophoresis |
2.4.1.18 | anion exchange chromatography |
2.4.1.18 | anion exchange column and hydrophobic column |
2.4.1.18 | chimeric and N-terminal truncated enzymes: DEAE-Sepharose Cl6B column, Bio-Gel P-200 and Gigapite column all at 4°C and pH 7.5. Site-directed mutant enzymes: chelating Sepharose FF column and DEAE-Sepharose CL-6B column, all at 4°C. |