EC Number   |
|---|
    2.3.3.21 | - |
| 2.3.3.21 | affinity chromatography using a Ni2+-NTA (Ni2+-nitrilotriacetate) Superflow column |
| 2.3.3.21 | by affinity chromatography using a Ni2+-NTA Superflow column, the N-terminal His6-tag is removed by thrombin digestion, subsequently an anion-exchange Q-column is applied |
| 2.3.3.21 | by using Ni-nitrilotriacetic spin columns |
| 2.3.3.21 | cells are centrifuged, pellet sonicated, resuspended in 0.1 M N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid buffer, pH 7.5 |
| 2.3.3.21 | full-length and mutant CMS |
| 2.3.3.21 | recombinant CimA 26.5fold from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation and heat treatment at 60°C for 10 min, followed by gel filtration |
| 2.3.3.21 | recombinant His6-tagged enzyme 14.3fold from Escherichia coli by nickel affinity chromatography to homogeneity |
| 2.3.3.21 | using a Ni2+-NTA Superflow column and an anion-exchange Q-column, the tag is removed by cleavage with thrombin |
| 2.3.3.21 | with Ni-nitrilotriacetic acid spin columns |
