EC Number |
---|
1.2.1.104 | - |
1.2.1.104 | alpha and beta subunits purified from enzyme complex after gel filtration in the presence of 2 M KI followed by chromatography on hydroxyapatite in the presence of 8 M urea |
1.2.1.104 | isolation of EC 1.2.4.1 after elastase treatment of the complex and gel filtration |
1.2.1.104 | Ni2+-Sepharose column chromatography |
1.2.1.104 | purification of pyruvate dehydrogenase complex including pyruvate decarboxylase E1 |
1.2.1.104 | purification procedure for the 2-oxoglutarate dehydrogenase and the pyruvate dehydrogenase complexes from mitochondria. After fractionated precipitations with polyethylene glycol, elimination of thiol proteins, and gel-filtration chromatography, the resulting preparations contain both activities. Covalent chromatography on thiol-activated Sepharose CL-4B allows the specific binding of the 2-oxoglutarate dehydrogenase complex activity in the presence of 2-oxoglutarate, the pyruvate dehydrogenase complex activity is retained in the presence of pyruvate |
1.2.1.104 | purification scheme for the pyruvate dehydrogenase complex directly from body wall muscle which yields a fully activated pyruvate dehydrogenase complex with substantial PDHa kinase activity |
1.2.1.104 | pyruvate dehydrogenase can be resolved from complex by incubation with 8 M urea, 4 M guanidinium chloride, 100 mM glycine/NaOH, pH 9.0 followed by gel filtration |
1.2.1.104 | pyruvate dehydrogenase complex composed of EC 1.2. 4.1, EC 2.3.1.12, EC 1.8.1.4 |
1.2.1.104 | pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 |