EC Number |
---|
1.17.1.4 | - |
1.17.1.4 | 529fold purification, several (NH4)2SO4 precipitation steps |
1.17.1.4 | adenine may play a role in preventing the dehydrogenase to oxidase conversion during extract preparation, storage, overnight dialysis and heat treatment |
1.17.1.4 | after expression in Escherichia coli |
1.17.1.4 | ammonium sulfate precipitation, phenyl-Sepharose column chromatography, DEAE-10 Macro-Prep MP10 column chromatography, and Bio-Prep SE-1000/17 column chromatography |
1.17.1.4 | during purification conversion from dehydrogenase type D to oxidase type O, reversible by dithioerythritol |
1.17.1.4 | from liver |
1.17.1.4 | highly active bovine XOR in its XDH form is prepared by folate affinity chromatography |
1.17.1.4 | immobilized on n-octylamine-substituted Sepharose 4B |
1.17.1.4 | in presence of dithiothreitol |