EC Number   |
|---|
    1.13.12.7 | - |
| 1.13.12.7 | a more efficient method for the purification and concentration of luciferase using aqueous two-phase extraction (ATPE) is designed. Downstream processing of luciferase from Photinus pyralis is carried out using polymer/salt aqueous two phase system (ATPS) at 4°C. The enzyme is observed to preferentially partition to the polyethylene glycol (PEG) rich top phase. The best results of purification (13.69 fold) and enzyme activity recovery (118.34%) are observed in the system containing 4.0% (w/w) PEG (1500) and 20.5% (w/w) (NH4)2SO4 with a phase volume ratio of 0.21 |
| 1.13.12.7 | affinity chromatography |
| 1.13.12.7 | agarose-Ni2+ column chromatography |
| 1.13.12.7 | alkyl-substituted Sepharose 4B affinity chromatography |
| 1.13.12.7 | ammonium sulfate fractionation and gel filtration of the wild-type and several mutants |
| 1.13.12.7 | ammonium sulfate fractionation and ion-exchange chromatography of the wild-type and several mutants |
| 1.13.12.7 | ammonium sulfate precipitation, gel filtration, hydroxyapatite HPLC |
| 1.13.12.7 | chromatography on DEAE-cellulose |
| 1.13.12.7 | gel filtration |
