EC Number   |
Application |
Reference |
|---|
    1.1.3.15 | agriculture |
inoculation of plants with Pseudomonas syringae increases photorespiration rate and expression of glycolate oxidase (GOX2), serine glyoxylate aminotransferase (SGT) and serine hydroxyl methyltransferase (SHMT1). Silencing of GOX2, SGT or SHMT1 genes in tomato decreases photorespiration but increases susceptibility to Pseudomonas syringae, whereas transient overexpression of GOX2, SGT or SHMT1 in tobacco increases basal defence. Salicylic acid signalling is involved in GOX2-mediated, SGT-mediated and SHMT1-mediated defence. H2O2 pretreatment remarkably alleviates the GOX2 silencing-induced depression in basal defence and salicylic acid signalling |
763562 |
| 1.1.3.15 | analysis |
construction of a D-Lactate electrochemical biosensor. The electrode for detection of D-lactate is prepared by immobilizing dye-linked D-LDH and multi-walled carbon nanotube within Nafion membrane. The electrode response to D-lactate is linear within the concentration range of 0.03-2.5 mM, and it shows little reduction in responsiveness after 50 days |
761556 |
| 1.1.3.15 | analysis |
development of a lactate biosensor through immobilization of lactate oxidase in an albumin and mucin composed hydrogel by gluitaraldehyde cross-linking and trapping between two polycarbonate membranes. Hydrogen peroxide produced is detected on a platinum electrode. The response time of the sensor to 0.010 mM lactate requires 90 s to give a 100% steady-state response of 0.079 microA. Linear behavior is obtained between0.7 microM and 1.5 mM. The detection limit calculated from the signal to noise ratio was 0.7 microM |
690071 |
| 1.1.3.15 | diagnostics |
lactate biosensor (FIA amperometric detection), tested with beer samples, detection limit: 0.84 micromol/l, three-electrode cell with AG/AGCl, NaCl reference electrode, platinum wire, glassy carbon electrode, the latter layered with Prussian Blue film of K3Fe(CN)6, Fe(III), KCl, cetyltrimethylammonium bromide, pH 1.7 maintained with HCl, lactate oxidase immobilized onto the Prussian Blue-modified glassy carbon electrode, enzyme layer covered by a Nafion-ethanol solution, storage in phosphate buffer, pH 6.9 |
699088 |
| 1.1.3.15 | diagnostics |
lactate biosensor development based on an enzymatic recognition system using lactate oxidase and a transduction system based on laminol, peroxidase from Arthromyces ramosus and metallic aluminum, immobilized in a plastic support by a polyion complex membrane prepared from poly-L-lysine hydrobromide and poly(sodium 4-styrenesulfonate) |
695568 |
| 1.1.3.15 | diagnostics |
the metabolic gene HAO2 is downregulated in hepatocellular carcinoma and predicts metastasis and poor survival. Dysregulation of HAO2 is a very early event in the development of hepatocellular carcinoma and it may represent a useful diagnostic and prognostic marker for human hepatocellular carcinoma |
745481 |
| 1.1.3.15 | drug development |
in humans the enzyme is a potential drug target for treatment of primary hyperoxaluria, a genetic disorder where overproduction of oxalate results in the formation of kidney stones |
698951 |
| 1.1.3.15 | drug development |
isozyme Hao2 is a target for drug development in high blood pressure |
723811 |
| 1.1.3.15 | medicine |
application of oan in vivo CRISPR/Cas9-mediated substrate reduction therapy to treat primary hyperoxaluria type I that results in excessive hepatic oxalate production causing end-stage renal disease. A single systemic administration of an AAV8-CRISPR/Cas9 vector targeting glycolate oxidase, prevents oxalate overproduction and kidney damage, with no signs of toxicity in Agxt1-/- mice |
763509 |
| 1.1.3.15 | medicine |
decrease of enzyme activity upon oxidative stress induced by glutathione depletion or postischemic perfusion, down-regulation of enzyme as mechanism to prevent excessive H2O2 formation in liver peroxisome |
655694 |
