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EC Number	Organic Solvent	Commentary	Reference
1.5.1.3	dimethyl sulfoxide	less than 3% inhibition in presence of dimethylsulfoxide at concentrations used for inhibitor dissolvation	686360
1.5.1.3	DMSO	irreversibly impairs the enzymatic activity at 30% v/v	743663
1.5.1.3	urea	dihydrofolate reductase hvDHFR1 is ,12 kcal/mol more stable to urea denaturation than dihydrofolate reductase hvDHFR2. NaCl, KCl, and CsCl enhance the stability to urea denaturation	728128

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Release 2023.1 (January 2023)