EC Number   |
Organic Solvent |
Reference |
|---|
    1.1.1.2 | acetonitrile |
62% remaining activity at 10% after 72 h at 5°C, 34% at 5°C, rapid inactivation at 50°C and 30% |
722209 |
| 1.1.1.2 | DMSO |
78% remaining activity at 30% after 72 h at 5°C, inactivation within 24 h at 50°C and 30% |
722209 |
| 1.1.1.2 | isopropanol |
solvent-tolerant enzyme, highly efficient reduction of ketones by coupling with excess isopropanol to regenerate NADPH |
738511 |
| 1.1.1.2 | Methanol |
at 5°C and 50°C, 38% remaining activity at 30%, 67% at 10%, and 70% at 5% after 72 h, inactivation within 24 h at 50°C and 30% |
722209 |
| 1.1.1.2 | Methanol |
the purified enzyme does not oxidize methanol. The enzyme retains almost its full activity when the concentration of methanol is up to 5% (v/v). When the concentration of methanol is 40% (v/v) in the assay mixture, the enzyme activities remain at 30% and 15% of the full activity on both oxidation and reduction, respectively |
738511 |
| 1.1.1.2 | more |
longterm effects of organic solvents at 5°C and 50°C, overview. High concentration of salt, essential to maintain the enzymatic activity and structural integrity of the halophilic enzyme under standard conditions may be partially replaced by DMSO and MeOH. Presence of organic solvents does not induce gross changes in substrate specificity |
722209 |
| 1.1.1.2 | tetrahydrofuran |
at 5°C, 32% activity remaining after 24 h, 7% after 72 h at 5% THF, 12% remaining activity at 10% THF after 24 h, inactivation within 72 h at 10% THF, and within 24 h at 30%THF |
722209 |
