EC Number   |
Natural Substrates |
|---|
   2.1.1.35 | more |
the enzyme emerged through an ancient horizontal transfer of an RNA (uracil, C5)-methyltransferase-like gene from bacteria to archaea |
| 2.1.1.35 | S-adenosyl-L-methionine + tRNA containing uridine at position 54 |
- |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
- |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
in vivo present both as 42 kDa polypeptide and as covalent TrmA/RNA complex, to equal amounts, both with methylating activity, in vivo the m5U54 modification confers a small selective growth advantage, two different catalytic activities: synthesis of m5U54 and an unknown essential function, may be associated with binding RNA, either during a maturation process of the rRNA and/or tRNA or as a regulatory device during the assembly and maturation of the translational apparatus such as an RNA chaperone |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
specific reaction in maturation of tRNA |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
tRNA modifying enzyme |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
role per se in tRNA maturation, stabilization of tRNA, early event in tRNA maturation: intron-containing pre-tRNAs contain m5U |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
enzyme-tRNA complexes may exist as dihydrouridine intermediates and may serve as reservoirs of RUMT-tRNA complexes primed for methylation |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
involved in biosynthesis of all tRNA chains, association of enzyme to tRNA may be of regulatory significance |
| 2.1.1.35 | S-adenosyl-L-methionine + uridine54 in tRNA |
specific inhibition of enzyme by ethionine in vivo |
