EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
1.6.5.4 | -999 |
- |
more |
kinetics |
654107 |
1.6.5.4 | 0.0006 |
- |
NADH |
with substrate monodehydroascorbate |
655510 |
1.6.5.4 | 0.001 |
- |
NADPH |
pH 7.8, temperature not specified in the publication |
765560 |
1.6.5.4 | 0.0012 |
- |
NADH |
with ferricyanide as substrate |
655510 |
1.6.5.4 | 0.0012 |
- |
semidehydroascorbic acid |
- |
392726 |
1.6.5.4 | 0.0013 |
- |
ferricyanide |
- |
655510 |
1.6.5.4 | 0.00144 |
- |
monodehydroascorbate |
native wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C |
712176 |
1.6.5.4 | 0.0015 |
- |
monodehydroascorbate |
recombinant wild type enzyme, in 50 mM Tris-HCl (pH 7.5), at 25°C |
712176 |
1.6.5.4 | 0.00151 |
- |
monodehydroascorbate |
mutant enzyme C117S, in 50 mM Tris-HCl (pH 7.5), at 25°C |
712176 |
1.6.5.4 | 0.00155 |
- |
monodehydroascorbate |
mutant enzyme C117A, in 50 mM Tris-HCl (pH 7.5), at 25°C |
712176 |